The biochemical examination of semen is currently carried out. At present, there are many projects such as fructose, acidic phosphatase, citric acid, protein, lactate dehydrogenase X and trace elements.During the biochemical examination of semen, the semen specimen should be placed in the water bath box of 35 ℃ ～ 37 ℃. After liquefaction, take 1.2 to 1.5ml centrifugal for 15 minutes.
(1) Determination of fructose
Generally uses a phenylbhenol, with a normal value of 256 ± 104 mg/dl.Fructose is mainly secreted by seminal vesicles, which is the main source of sperm energy metabolism, which is related to sperm activity.Fructose is zero, and the lack of seminal sacs should be considered.When the semen is inflammated, the fruit sugar content decreases.In addition, insufficient male hormones and decreased fructose content during old age, and higher fruit sugar content in the semi -slurry during diabetes.
(2) Acidal phosphatase
Merit with & beta; glycerin phosphate.The normal value is 882 ± 412, a BRIC unit/ml.This enzyme mainly comes from the prostate.It is related to the vitality and metabolism of sperm.At the time, the content of acidic phosphatase in the sperm paste decreases; early prostate cancer or benign prostate hypertrophy, and the content of acid phosphalaase often increases.
(3) Sperm -specific enzymes -lactate dehydrogenase X (LDHX) and LDH homogeneous enzymes have a relative tissue specificity and have a certain relationship with the function of raw sperm.Men's infertility patients with too many cottonseed oil are reduced.
(4) protein
Merit with dual urination method.Normal people are 3.6 ± 0.8g/dl.The protein in semen is mainly from seminal vesicles and prostate, which can increase sperm vitality and protect sperm from the harmful effect of the environment.
(5) Trace elements
It plays an important role in male reproductive and reproductive endocrine functions.The zinc in normal semen is 130 ± 56 & mu; g/ml, copper: 1.85 ± 0.15 & mu; g/ml, iron: 0.81 ± 004 & mu; g/ml.Zinc in semen can affect sperm metabolism, and proportional to sperm activity and density, and can indirectly affect gonad by affecting the pituitary gland. When zinc is insufficient, the abolition of the pituitary gonad hormone is reduced, the gonads are dysplasia, or the gonad is dysplasia, or the gonad is dysplasia, or the gonad is dysplained, or the sexual gonad is dysplained, or the sexual gonad is dysplained, or the sexual gonad is dysplained, or the sexual gonad is dysplasia, or the sexual gonad is dysplained, or the gonad is dysplasia, or the gonad is dysplained, or the gonad is dysplained, or the gonad is dysplasia. The reproductive and endocrine dysfunction of the gland has disorders.Copper can affect sperm survival and activity. The copper concentration in semen is high and sperm activity is poor.Conversely, it is good.The density of sperm and semen in the iron is obviously related to the semen. The sperm density in the sperm containing the sperm is high, otherwise, the sperm density is low.
(6) Clear citric acid
Through FURTHHHERMANN reaction measurement, the normal value is 56 ± 2.0mg/dl, mainly from the prostate, regulating the calcium ion concentration in the semen, which affects the liquefaction of semen, and has the effect of prostate phosphatase activation agent, which affects the vitality of the sperm.When prostate has inflammation, the citric acid content is significantly reduced.
(Editor in charge: Chen Bong)
